Basal Medium

B group : endothelial basal medium with VEGF and bFGF ;

B组：在内皮基础培养基中添加生长因子VEGF、bFGF；

C group : endothelial basal medium .

C组：仅应用内皮基础培养基培养。

Using 1 / 2MS basal medium with 3 % sucrose .

基本培养基为1/2MS，蔗糖浓度为3%。

B5 basal medium was more effective in inducing callus than MS basal medium .

B5培养基较MS培养基有利于愈伤组织诱导。

The effect of basal medium was modified MS > QL > WPM .

基本培养基的效果依次为改良MS>QL>WPM。

Somatic embryos germinated on MS basal medium free of plant growth regulators developed into plantlet .

发育到子叶期的体细胞胚在不加任何激素的MS培养基上就可以萌发，再生成完整植株。

In vitro propagation of garlic was studied by using regular varieties as material and B5 as the basal medium .

以常规大蒜栽培品种为试材，B5为基本培养基，研究了利用脱毒大蒜萌生幼苗的离体繁殖技术。

The results showed that the effects of basal medium , IBA and dark or light culture were significant at1 % level .

结果表明基本培养基、IBA和光暗培养是影响试管苗产生不定根的主要因素。

The effect of PAA stimulating one-step seedling formation depends on the prescription of hormone concentration , basal medium .

试验证明PAA对一步成苗的促进效应与各种激素浓度配比和基本培养基的种类有关，与基因型的关系不明显。

1 / 2 MSwas the suitable basal medium .

诱导生根的基本培养基以1／2MS为宜。

The B5 and MS basal medium were fit to cell growth and the most favorable initial pH value of medium was 6.0 .

B5和MS基本培养基均适合细胞的生长。培养基的最佳初始培养基pH值为6.0。

Callus formation frequency as well as plantlet regeneration frequency was dependent on the composition of basal medium and the concentration of2,4-D.

胚性愈伤诱导率和植株再生率与培养基的种类、培养基中2，4-D的浓度、小麦基因型及诱导和分化培养基中激素种类和浓度等有关。

The B5 basal medium is best for cell growth ; 3.The biggest multiplication rate 5.1 was obtained at the pH value of 6.5 ;

培养基的初始pH值为6.5时，丹参不定根的生长情况最好，其增殖倍数为5.1；

Compared with MS ( 1 / 2N ), MS basal medium was more beneficial to forepart subculture micropropagation .

与MS（1/2N）和1/2MS相比，MS基本培养基更有利于组培苗早期继代增殖。

The results showed that the effective order of three factors on regeneration was basal medium , 6-BA and NAA . Empty in the central of the stem ;

结果表明，3个因子对长富2号黄化茎段再生的影响程度依次为基本培养基>6-BA>NAA；

One-half MS basal medium added 0.1-0.2mg/lNAA or IAA can make the root long and strong . The activated charcoal is good to root formation and growth .

生根培养时，以1/2MS为基本培养基，附加0.1-0.2mg/lNAA或IAA可使根长的长而壮，活性炭对根的生长有利。

The results were as following : ( 1 ) When callus development was initiated with the mature embryo of Douglas-fir , the basal medium had some influence .

其主要研究结果如下：（1）以美国花旗松成熟合子胚为外植体进行愈伤组织的诱导时，基本培养基对其有一定的影响。

The results showed that the effect of the 4 factors on subculture micropropagation was in the order of basal medium , ZT , IAA and BA .

方差分析结果显示，基本培养基对磨盘柿继代增殖的影响极为显著，ZT的作用次之，IAA作用较弱，BA最弱。

Chlorella cells containing polyphosphate grew in phosphate-free Basal medium , and the total cellular phosphorus content was 1.0 mg / g at the end of the cultivation .

将含多聚磷的小球藻细胞转移到无磷培养基中继续培养，小球藻可以利用胞内储存的多聚磷继续生长，直到细胞内总磷含量为1.0mg/g时，生长基本停止。

This experiment screened out the optimal culture date , relative optimal basal medium components with concentrations of several kinds of hormones , and obtained a set of hybridization materials from embryo rescue .

筛选出了早熟葡萄胚挽救的最优培养时期、相对较优的基本培养基成分及几种激素浓度，积累了一批胚抢救杂交试材。

The 6-BA , basal medium , IBA and sucrose were used to compare their effect on adventitious bud regeneration from leaf of raspberry by using the orthogonal experiment design .

利用正交设计试验法研究了基本培养基、6-BA、IBA、和蔗糖4个因素及其组合对树莓叶片再生的影响。

1 week after primary culture , in vitro spinal cord derived NSCs clone was obtained . Feta calf serum of 0.1 volume fraction was added in the basal medium .

原代培养1周后，获取体外稳定增殖的鼠脊髓源性神经干细胞克隆，在基础培养基中加入体积分数为0.1的胎牛血清作为对照。

The percentage of yeast extract of the nitrogen source during the growth phase and in the basal medium during the phase of induction and the time and condition that the induction starts are effective .

结果表明，基础料和补充氮源中酵母膏的比例、诱导时机和乳糖流加方式等对表达量的提高有明显的影响。

Adventitious buds were regenerated from epicotyl segments in improved MS basal medium , and the addition of 6-BA and IAA in the media influenced the buds regeneration .

上胚轴在改良的MS基本培养基中能形成不定芽，6-BA的加入有利于不定芽的再生，IAA的加入则不利于不定芽的再生。

Basal medium was composed of penicillinum , phytomycin , amphotericin B and DMEM / F12 containing B27 annex solution of 0.02 volume fraction .

基础培养基组成：含青霉素、链霉素、两性霉素B及体积分数为0.02B27添加液的DMEM/F12。

During the shoot proliferation , we found that MS basal medium was more suitable for growth and subculture of the shoots than MS-R , Gs and H basal mediums .

继代增殖培养时，选用MS为基本培养基效果优于MS-R、GS和H培养基。

When NAA and BA are added together in the basal medium ( NAA 0.5 mg / 1 , BA 1.0 mg / 1 ) , the seeds germinate and grow faster .

培养基中同时附加NAA及BA，在适宜的比例下（NAA0.5毫克/升，BA1毫克/升），种子萌发生长得较快。

Method : MS medium was used as basal medium and BA and NAA were supplemented to find out the optimal hormone combinations for adventitious buds initiation , adventitious bud multiplication and rooting .

方法：以MS培养基为基本培养基，通过研究植物生长物质BA和NAA对丛生芽的分化、丛生芽的增殖和生根的影响找出相应的最佳生长物质组合。

The stem explants of Fortunella margarita were cultured on MS basal medium supplemented with 1.0 mg / 1 BA and 0.1mg/1 IBA for induction of callus and bud formation .

用金桔茎段为外植体，培养在附加1.0毫克/升BA和0.l毫克/升IBA的MS培养基上，诱导愈伤组织和芽形成。

At present , medium used in the in vitro maturation system is adding serum , albumin , all kinds of cytokines and hormone to basal medium , in a certain extent , which improved the efficiency of in vitro maturation .

目前体外成熟体系中所用的培养液是向基础培养基中添加血清、白蛋白、各种细胞因子和激素等，在一定程度上提高了体外成熟的效率。